WildlifeSNPits

Congratulations to SNPits blogger Anna on her new paper (sub) on DNA barcoding for invasive red foxes (Vulpes vulpes) in Tazmania. She wrote an accompanying blog post about the paper.

From Benchtop to Desktop: Important Considerations when Designing Amplicon Sequencing Workflows
Amplicon sequencing (PCR and sequencing of specific target genes) using high throughput sequencing platforms has become an important tool for biodiversity assessments, dietary studies etc. Quite a bit of attention has been paid to the pros and cons of the different sequencing platforms used and the bioinformatic approaches to data analysis, but there has been less focus in the literature on study design and the laboratory steps leading up to and including library preparation. This is unfortunate, because, as in the words of Murray et al. “No amount of high-end bioinformatics can compensate for poorly prepared samples…”. This paper provides a great overview of amplicon library preparation…

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